Saracens and England's Farrell will lead the side out at the Marvel Stadium in Melbourne on what will be his 20th Lions appearance. Farrell, who lines out at inside centre and will form a partnership with Leinster Rugby and Ireland's Jamie Osborne.
Toulon and Scotland scrum-half Ben White is partnered at half-back by Northampton Saints and England's Fin Smith.
Toulouse and Scotland fullback Blair Kinghorn returns from injury and forms an all-Scotland back three alongside Edinburgh Rugby duo Darcy Graham and Duhan van Der Merwe.
Up front, Scotland and Edinburgh Rugby's Pierre Schoeman packs down alongside Saracens and England's Jamie George and Connacht Rugby and Ireland's Finlay Bealham.
Leinster Rugby and Ireland's James Ryan is partnered in the row by Glasgow Warriors and Scotland's Scott Cummings.
In the back row, Ospreys and Wales' Jac Morgan will wear the No.6 jersey, Leinster Rugby and Ireland's Josh van der Flier will wear the No. 7 jersey and Northampton Saints and England's Henry Pollock will pack down at No.8.
"This fixture gives players another opportunity to put their hand up for selection for the final two Tests," said Farrell.
"The coaches' minds are open when it comes to selection, they have to be with so many players performing and pushing for places and we want that to continue for the remaining two Tests.
This group know the level of performance that we will require against a First Nations Pasifika team that is packed with talent and power. "
British and Irish Lions - 15 Blair Kinghorn, 14 Darcy Graham, 13 Jamie Osborne, 12 Owen Farrell (c), 11 Duhan van Der Merwe, 10 Fin Smith, 9 Ben White, 8 Henry Pollock, 7 Josh van der Flier, 6 Jac Morgan, 5 Scott Cummins, 4 James Ryan, 3 Finlay Bealham, 2 Jamie George, 1 Pierre Schoeman.
Replacements: 16 Ewan Ashman, 17 Rory Sutherland, 18 Tom Clarkson, 19 Gregor Brown, 20 Ben Earl, 21 Alex Mitchell, 22 Marcus Smith, 23 Garry Ringrose.
Wallabies back Harry Potter has strongly dismissed former England and British & Irish Lions coach Sir Clive Woodward's accusation that Australian rugby is plagued by a "losing mentality."
The British and Irish Lions narrowly avoided a slip-up in their final midweek tour match, scraping past a spirited First Nations & Pasifika XV 24-19 in Melbourne on Tuesday.
First Nations and Pasifika XV head coach Toutai Kefu has named Kurtley Beale as captain of his matchday squad for Tuesday's clash against the British and Irish Lions.
The British and Irish Lions went ahead in the three-match series against Australia after a comfortable 27-19 victory at Suncorp Stadium.
