The 26-year-old midfielder has been sidelined for much of the season with injury but returned to play four league games and the Nedbank Cup last 32 victory over Free Agents where he was named Man of the Match.
But he broke down again on the hour mark in the Soweto Derby defeat to Orlando Pirates and subsequently missed the 2-2 draw with AmaZulu at the FNB Stadium.
Ahead of this weekend's game, Nabi said on iDiski Times: "George Matlou was injured [from the Soweto Derby] and was not available against AmaZulu and will not be available for our next game [against Stellenbosch]."
The head coach added: "For the second round of the league, we have to take things game by game and try to do the best performance each and every game fighting for three points, if we win, while not conceding goals, it would be ideal.
"But the most important thing is to build a very competitive team, a consistent one that gets performances and results, the processes of building a very good team passed by every game."
Nabi will, however, have Njabulo Blom available for selection after he completed his one-match suspension.
Blom missed Chiefs' Usuthu stalemate after receiving his fourth yellow card against Pirates.
Orlando Pirates coach Abdeslam Ouaddou has challenged Sebastian Pedersen to raise his fitness levels after getting his first glimpse of the Norwegian striker in competitive action.
Mamelodi Sundowns coach Miguel Cardoso has defended his decision to hand Lebo Mothiba a rare start against Golden Arrows, insisting the striker's performances in training warranted an opportunity in the MTN8 semi-final.
Deon Hotto believes Orlando Pirates are in a strong position to reach another MTN8 final after securing a narrow first-leg advantage over Sekhukhune United.
Sports Minister Gayton McKenzie has threatened to escalate South Africa's stalled Video Assistant Referee (VAR) project to FIFA as frustration grows over the technology's continued absence from the Premier Soccer League.
