The 26-year-old midfielder has been sidelined for much of the season with injury but returned to play four league games and the Nedbank Cup last 32 victory over Free Agents where he was named Man of the Match.
But he broke down again on the hour mark in the Soweto Derby defeat to Orlando Pirates and subsequently missed the 2-2 draw with AmaZulu at the FNB Stadium.
Ahead of this weekend's game, Nabi said on iDiski Times: "George Matlou was injured [from the Soweto Derby] and was not available against AmaZulu and will not be available for our next game [against Stellenbosch]."
The head coach added: "For the second round of the league, we have to take things game by game and try to do the best performance each and every game fighting for three points, if we win, while not conceding goals, it would be ideal.
"But the most important thing is to build a very competitive team, a consistent one that gets performances and results, the processes of building a very good team passed by every game."
Nabi will, however, have Njabulo Blom available for selection after he completed his one-match suspension.
Blom missed Chiefs' Usuthu stalemate after receiving his fourth yellow card against Pirates.
Orlando Pirates secured their place in a second cup final this season after edging Richards Bay FC 1-0 in the Carling Knockout semi-final at the Moses Mabhida Stadium on Saturday.
Marumo Gallants head coach Alexandre Lafitte is keeping his focus firmly on Sunday's Carling Knockout Cup semi-final against Golden Arrows, as the young French tactician looks to guide his side to a historic final appearance.
Golden Arrows head coach Manqoba Mngqithi says he is expecting a completely different challenge when his side meets Marumo Gallants again in the Carling Knockout Cup semi-final at the King Goodwill Zwelithini Stadium on Sunday.
As Abdeslam Ouaddou gears up to guide Orlando Pirates into yet another cup final, the Moroccan coach has opened up about the relentless pressure that comes with managing one of Africa's biggest football clubs.
