The 34-year-old Al-Hilal defender picked up a thigh injury in training and has not played a competitive game since featuring in the Saudi Pro League victory over Al-Kholood at the beginning of April.
He has been in recovery for the past month but is not yet back to full fitness as the Lions of Teranga prepare to face France, Norway and Iraq in a tricky Group I.
Koulibaly was, however, included in Senegal coach Pape Thiaw's preliminary 28-man squad for the World Cup and is expected to make the final cut.
Fresh from the controversy surrounding their Africa Cup of Nations 'win', that saw the nation stripped of their title and handed to hosts Morocco, the country beat Peru 2-0 and the Gambia 3-1 in international friendlies.
They face the United States of America and Saudi Arabia before kicking off their World Cup campaign.
Thiaw said he is positive that Koulibaly will be fit to face France on Tuesday 16 June.
"We are monitoring his progress in collaboration with the medical staff. We will communicate on this at the appropriate time, when we have more certainty. But the progress is positive.
"We hope he will be ready. I think so and I believe he will be. We have confidence in our medical staff and our physical trainers," said the head coach on Africa Soccer.
Christian Pulisic delivered a goal and assist as the United States secured a 3-2 friendly victory over Senegal, with head coach Mauricio Pochettino praising the "commitment" and "attitude" of his squad ahead of the 2026 FIFA World Cup.
Bafana Bafana's delayed journey to the 2026 FIFA World Cup is set to begin on Monday after all members of the playing squad received their visas, ending an administrative issue that forced the team to postpone its scheduled departure.
Luis Suarez has been omitted from Uruguay's squad for the 2026 World Cup, with head coach Marcelo Bielsa opting for a younger, high-energy group despite leaving out one of the country's greatest-ever players.
The South African Football Association say they are 'working around the clock' to get Bafana Bafana to Mexico after visa issue delayed the national's team's departure.
