Both teams have been struggling in their respective leagues adding extra weight to the outcome of the encounter as they go in search of positive momentum.
SuperSport United secured their place in this stage of the competition with a comfortable 2-0 victory over Magesi in the Round of 32.
Despite their cup success, the team has been struggling in the Betway Premiership, with their most recent league outing ending in a 1-1 draw against Sekhukhune United.
Looking to turn their fortunes around, they will aim to replicate their previous cup performance against Spurs.
Cape Town Spurs, currently competing in the Motsepe Foundation Championship, find themselves in a difficult position, sitting 15th in the relegation zone.
Their last league match saw them suffer a heavy 3-0 defeat against Black Leopards, further compounding their struggles.
However, they will take confidence from their 3-1 win over Venda Football Club in the previous round of the Nedbank Cup, as they attempt to spring a surprise against top-flight opposition.
Kaizer Chiefs forward Wandile Duba is refusing to let a lack of playing time derail his ambitions, despite facing one of the most competitive attacking line-ups in the Premier Soccer League this season.
Cape Town Spurs say the future of their club and academy hang in the balance after SAFA's ruling on the Asanele Velebayi and Luke Baartman case.
Cape Town Spurs have issued a statement accepting the outcome of an arbitration ruling by Advocate Hilton Epstein SC, which allows Asanele Velebayi and Luke Baartman to register with Kaizer Chiefs.
The dispute involving Liam Bern, Cape Town Spurs, and AmaZulu will be heard by the PSL's Dispute Resolution Chamber (DRC) on Tuesday, according to Sportswire.
