The first Test between these two sides in Osaka last week was a close affair, with the Wallabies holding on for a 35-32 victory, but after a competitive first half in Queensland on Saturday, the hosts ran away with the contest in the second.
The Wallabies only led 21-17 at half-time after Tupou's second try on the stroke of the break, but ran in five unanswered tries after the interval to complete a seven-try victory.
Josh Nasser, Angus Bell, debutant Massimo De Lutiis, Charlie Cale, Harry Wilson and Brandon Paenga-Amosa also crossed, with Ryan Lonergan adding five conversions.
The result extended Australia's unbeaten record against Japan to nine Tests and provided another boost for new coach Les Kiss, who has overseen consecutive wins over the Brave Blossoms following last week's victory in Osaka.
The Wallabies continued their impressive start to the Les Kiss era with a hard-fought 27-21 victory over Argentina in Jujuy, surviving a fierce second-half fightback from Los Pumas.
Les Kiss began his tenure as Wallabies head coach with a hard-fought 35-32 victory over Japan in Osaka, but Australia were made to work for every point.
Despite being 38 years of age, Quade Cooper has thrown his hat into the ring to be considered as an option at flyhalf for the Wallabies ahead of the 2027 Rugby World Cup.
Australia veteran James O'Connor was linked to a move to the Sharks, but has ultimately decided to stay put at home following personal news.
