Borthwick's men come into the Championship as red-hot favourites following a run of 11-straight Test victories in 2025, and will be hoping to claim their first title since 2020 in the process.
Within the uncapped cohort, Bath tighthead Vilikesa Sela and Northampton Saints loosehead Emmanuel Iyogun come into the wider training group, with the front-row in the midst of a major injury crisis. Elsewhere, Exeter Chiefs back-rower Greg Fisilau is also in line to win his first senior cap, after cracking the initial squad.
They are not the only fresh faces among the wider front-row unit, either, with Trevor Davison also making a return to the squad, while Bevan Rodd also sees himself included.
The headline selection, however, is the return of Northampton full-back George Furbank, who comes into the group for the first time since last year's Championship. Crucially, he is included in the wider squad rather than the rehabilitation group, which suggests he is fit to play come the opening round against Wales.
Fin Baxter, Ben Curry, Ollie Lawrence, Tom Roebuck and Fin Smith will also travel with the squad to their training base in Girona to undergo rehabilitation.
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Elsewhere, Maro Itoje will once again captain England, after steering the side through a successful 2025.
Commenting on his selection, head coach Borthwick said: "We've picked a squad with a good balance of experience, leadership and exciting potential.
"The margins in the Six Nations are incredibly fine so our aim over the coming days is to come together quickly, prepare thoroughly, and make sure we're ready to perform.
"If we prepare well and keep demanding the highest levels from one another, it puts us in the strongest possible position when the Championship begins."
England Six Nations squad
Forwards: Ollie Chessum, Arthur Clark, Alex Coles, Luke Cowan-Dickie, Chandler Cunningham-South, Tom Curry, Theo Dan, Trevor Davison, Ben Earl, Greg Fisilau, Ellis Genge, Jamie George, Joe Heyes, Maro Itoje (c), Emmanuel Iyogun, Guy Pepper, Henry Pollock, Bevan Rodd, Vilikesa Sela, Sam Underhill.
Backs: Henry Arundell, Seb Atkinson, Elliot Daly, Fraser Dingwall, Immanuel Feyi-Waboso, George Ford, Tommy Freeman, George Furbank, Alex Mitchell, Cadan Murley, Max Ojomoh, Henry Slade, Marcus Smith, Ben Spencer, Freddie Steward, Jack van Poortvliet.
Bundee Aki has been hit with a suspension for verbal abuse and disrespect towards match officials, resulting in a lengthy suspension that will see him miss a large part of the upcoming Six Nations.
Ireland's Six Nations preparations have been rocked by the revelation that Bundee Aki didn't fly to Portugal with the squad on Tuesday due to disciplinary reasons.
France and La Rochelle prop Uini Atonio has been forced to retire from rugby after suffering a heart attack that has left him in intensive care and unable to continue his playing career.
Springbok head coach Rassie Erasmus says World Rugby is taking the prospect of a globally aligned rugby calendar very seriously.
