The tournament, featuring three double-header match days, will be staged at City Park at the end of May, offering head coach Kevin Foote and his staff a final chance to fine-tune their squad ahead of the global showpiece, which begins in Georgia at the end of June.
"We know game time out on the field in actual matches is crucial as we prepare for the Junior World Championship, and we are truly grateful for this opportunity," said Foote.
"All the players in our wider squad will be considered for these matches, and apart from game time, we would like to work on combinations as we build up towards the trip to Georgia in June.
"We’re also excited to build on our relationships with the three visiting nations. We’re in the same JWC pool as Georgia, while Fiji will no doubt also use this opportunity to fine-tune their preparations for the tournament.
"Chile is one of the up-and-coming junior teams, and they will host the World Rugby U20 Challenger Cup this year, so I’m sure they will be eager to impress on home soil."
The Junior Boks are currently preparing for the U20 Rugby Championship in Gqeberha, where they will face the U20 sides of Argentina, Australia, and New Zealand for southern hemisphere supremacy.