Litton has had a dismal run in ODIs recently - he hasn't reached a double-digit score in eight matches since December 2023.
Nurul Hasan, Jaker Ali and Shamim Hossain have all missed out on selection as the selectors as several changes were made to the group.
"Litton Das may not have produced his best in recent ODIs but you can't overlook the experience of nearly a hundred international matches in this format," Bangladesh chief selector Gazi Ashraf Hossain said.
"He has been working closely with the coaches on his game. We believe he will soon perform in the manner he is capable of.
"We are considering him as an option to strengthen the middle order. Soumya Sarkar, meanwhile, has been in excellent form at the international level."
Ashrad added: "We have provided opportunities to several players in the middle order, but unfortunately some have not done enough to secure their places," Ashraf said.
"With that in mind, Afif Hossain has been recalled. He has never been away from our plans. Afif brings valuable experience and is currently in good touch.
"Mahidul Islam Bhuiyan has been retained and this is another opportunity for him to fulfil the potential he has shown."
The ODI series against Pakustan will begin on March 11 and will run until March 15 with matches played in Mirpur.
Bangladesh ODI squad: Mehidy Hasan Miraz (captain), Soumya Sarkar, Saif Hassan, Tanzid Hasan, Najmul Hossain Shanto, Tawhid Hridoy, Litton Das, Afif Hossain, Mahidul Islam Bhuiyan, Rishad Hossain, Tanvir Islam, Mustafizur Rahman, Taskin Ahmed, Shoriful Islam, Nahid Rana
Shakib Al Hasan feels the relationship between the cricket boards of Bangladesh and India eventually be repaired following the Tigers' decision to withdraw from the T20 World Cup earlier this year.
Tawhid Hridoy expressed his delight after playing what he described as an "impact innings" to help Bangladesh beat New Zealand by six wicket in the first of three T20Is.
Captain Mehidy Hasan Miraz praised the "extraordinary" performance of seamer Mustafizur Rahman after Bangladesh sealed a 2-1 ODI series victory at home against New Zealand.
Nahid Rana took his second career five-wicket haul to help Bangladesh beat New Zealand by six wickets in Mirpur and level the three-match ODI series at 1-1.
