Flower had previously successfully been England head coach between 2009 and 2014 and has now carved a out a successful career on the T20 circuit, notably winning back-to-back IPL titles with Royal Challengers Bengaluru.
Flower was seen a leading candidate to return to the England fold with the ECB now searching for a mentor to take over in the red-ball format.
"The bottom line for me is that I'm very happy in the work that I'm doing at the moment... I'm really comfortable with what I'm doing," Flower said at a media for Hundred franchise London Spirit. "I have (ruled myself out), yes.
"I work for a couple of really good organisations. I've got really good teams around me and good people around me and I'm really comfortable with what I'm doing at the moment.
"I've worked with England before and I had an amazing time when I had the privilege to be England head coach and represent England as one of their leaders a number of years ago, and I remember those years really fondly, and it would be a privilege for me or anyone else to be the Test coach.
"But for me at this time, I'm really comfortable with what I'm doing. I'm going to stick with doing that."
Jonathan Trott, who has been linked with the England Test coaching job, will join the Ireland staff as a consultant ahead of their ODI series against Afghanistan next month.
India made a winning start to the one-day international series with a comfortable six-wicket victory over England at Edgbaston on Tuesday, led by captain Shubman Gill and all-rounder Axar Patel.
Brendon McCullum admits his results as head coach of England's Test side were not good enough and it was probably for the best that he was relieved of his duties in the longest format.
Brendon McCullum has been dismissed as England's Test head coach, bringing the curtain down on the Bazball era just weeks after Ben Stokes retired from international cricket.
