The 27-year-old right-back has travelled with his team to South Africa for the group stage encounter but will play no part in the match after a suspension by the Confederation of African football.
Lupopo secured their place in the group stage with victory over Orlando Pirates in the Second preliminary round.
Both teams had a player sent off in the tie, while Mukoko escaped punishment for a headbutt attempt on Pirates' Masindi Nemtajela and a stamp on Oswin Appollis.
He also celebrated wildly in front of the Pirates bench after the final whistle before a confrontation with Pirates captain Nkosinathi Sibisi.
CAF have since reviewed the footage and issued a two-match ban.
Saint-Eloi Lupopo assistant coach Yassine Salmi believes the team's growth and tactical maturity will be crucial as they prepare for another major test against Mamelodi Sundowns.
Mamelodi Sundowns get their CAF Champions League group stage campaign underway against DR Congo Linafoot side Saint-Eloi Lupopo on Saturday.
Chilean midfielder Marcelo Allende admits it's crucial for Mamelodi Sundowns to win their opening CAF Champions League group stage clash.
Mamelodi Sundowns midfielder Neo Maema has revealed that his decision to join Simba SC in Tanzania was driven by a desire for a fresh start outside South Africa.
