The 27-year-old was one of a number of players released by Amakhosi as they prepare their squad for the upcoming Betway Premiership campaign.
Amakhosi confirmed that they would not be renewing Matlou's contract following four seasons with the club since joining from the now defunct Swallows in July 2022.
Matlou made 46 appearances for Chiefs, providing three assists while playing a key role in the 2024/25 Nedbank Cup title win.
A club statement read: "Stellenbosch Football Club is delighted to announce the signing of midfielder George Matlou on a free transfer.
“Matlou, who most recently plied his trade with Kaizer Chiefs, has penned a long-term contract with Stellenbosch FC ahead of the 2026-27 season.”
Stellenbosch FC CEO Rob Benadie added: "George is a player we believe can add real value to our squad through his experience, technical ability, and understanding of the game.
"His pedigree speaks for itself, and we are naturally pleased that he has chosen to continue his career with Stellenbosch FC. We believe he can play an important role in helping us achieve our objectives this season."
Stellenbosch FC have continued to beef up their squad ahead of the new season with the signing of Cape Town-born New Zealand goalkeeper Nathan Garrow on a permanent transfer.
Stellenbosch's signing spree continues with the acquisition of experienced defender Terrence Mashego on a season-long loan from Mamelodi Sundowns.
Thabo Moloisane is delighted to finally be a Kaizer Chiefs player after completing his move from Stellenbosch.
Newly appointed Kaizer Chiefs coach Fernando da Cruz was denied victory in his first match in charge after Amakhosi were held to a 1-1 draw by Scottish side Rangers in a pre-season friendly in Spain on Sunday.
