Defending champions Orlando Pirates take on Baroka at the Orlando Stadium on Saturday 15 February while runners-up Mamelodi Sundowns host Mpheni Hone Defenders.
Elsewhere, Kaizer Chiefs welcome Chippa United at the FNB Stadium and Stellenbosch take on Polokwane City, while SuperSport United are at home to Cape Town Spurs.
Sekhuhkune United, meanwhile, face the winner of Royal AM or Milford, with that clash yet to take place with Thwihli Thwahla currently suspended.
Tickets for all fixtures will be available from Ticketpro outlets nationwide for R40.
All last 16 fixtures
Wednesday 12 February
SuperSport United v Cape Town Spurs
Lucas Moripe Stadium
Thursday 13 February
Stellenbosch v Polokwane City
Danie Craven Stadium
Friday 14 February
Durban City v TS Galaxy
Chatsworth Stadium
Saturday 15 February
Orlando Pirates v Baroka
Orlando Stadium
Royal AM/Milford v Sekhuhkune United
Harry Gwala Stadium/Princess Magogo Stadium
Marumo Gallants v AmaZulu
DR Molemela Stadium
Kaizer Chiefs v Chippa United
FNB Stadium
Sunday, 16 February
Mamelodi Sundiwns Mpheni Hone Defenders
Loftus Versfeld
Durban City head coach Pitso Dladla saluted a excellent season as his team claimed the Nedbank Cup trophy and a top eight finish to their Betway Premiership campaign.
Durban City will celebrate their Nedbank Cup victory with a trophy parade on Wednesday, the eThekwini Municipality have confirmed.
Kaizer Chiefs midfielder Thabo Cele has warned that his team may 'spoil the party' and beat Betway Premiership leaders Mamelodi Sundowns on Wednesday.
Darren Keet revealed his plans to retire after helping Durban City to victory in the Nedbank Cup final against TS Galaxy on Saturday.
