Defending champions Orlando Pirates take on Baroka at the Orlando Stadium on Saturday 15 February while runners-up Mamelodi Sundowns host Mpheni Hone Defenders.
Elsewhere, Kaizer Chiefs welcome Chippa United at the FNB Stadium and Stellenbosch take on Polokwane City, while SuperSport United are at home to Cape Town Spurs.
Sekhuhkune United, meanwhile, face the winner of Royal AM or Milford, with that clash yet to take place with Thwihli Thwahla currently suspended.
Tickets for all fixtures will be available from Ticketpro outlets nationwide for R40.
All last 16 fixtures
Wednesday 12 February
SuperSport United v Cape Town Spurs
Lucas Moripe Stadium
Thursday 13 February
Stellenbosch v Polokwane City
Danie Craven Stadium
Friday 14 February
Durban City v TS Galaxy
Chatsworth Stadium
Saturday 15 February
Orlando Pirates v Baroka
Orlando Stadium
Royal AM/Milford v Sekhuhkune United
Harry Gwala Stadium/Princess Magogo Stadium
Marumo Gallants v AmaZulu
DR Molemela Stadium
Kaizer Chiefs v Chippa United
FNB Stadium
Sunday, 16 February
Mamelodi Sundiwns Mpheni Hone Defenders
Loftus Versfeld
Kaizer Chiefs forward Wandile Duba is refusing to let a lack of playing time derail his ambitions, despite facing one of the most competitive attacking line-ups in the Premier Soccer League this season.
The fixtures for the opening round of the 2025/26 Carling Knockout have been released by the Premier Soccer League.
Cape Town City chairman John Comitis has shared his thoughts on Khanyisa Mayo's shock return to South African football, while revealing how Kaizer Chiefs initially missed out on the striker before his move to Algeria.
Kaizer Chiefs defender Aden McCarthy is focused on achieving results as a team and downplayed the hype following his impressive start to the 2025/26 season.
