The French defender suffered a distal biceps femoris injury in his left thigh during the final minutes of the first half in Barcelona's recent clash with Inter Milan.
A key figure under Hansi Flick, the former Sevilla star expressed his frustration at the untimely injury during a critical phase of the season.
"I'm gutted to be sidelined at this stage of the season when everything is on the line, but sometimes you can't control these things," Jules Kounde said in a heartfelt post on his social media.
The 25-year-old was visibly distraught as he left the Montjuic stadium on Wednesday, grappling with the weight of this major setback.
Jules Kounde has been a near-ever-present figure for Barcelona, featuring in 53 matches this season. His only absence came in a La Liga fixture against Mallorca, a tactical decision by Flick to ensure he was fresh for the Copa del Rey final.
In that match, Kounde proved his worth, scoring the decisive goal to secure the trophy.
With the right-back now sidelined, Flick faces the challenge of reshaping his defence for these crucial encounters. Ronald Araujo and Eric Garcia are the leading candidates to fill the void on the right side of the backline.
"I have full confidence that this team will get the job done, no matter who's on the pitch. Thank you all for your support, it means the world," Jules Kounde added in his post, which drew supportive replies from teammates like Alejandro Balde and former Barcelona striker Memphis Depay, alongside other football figures.
With the La Liga title race heating up and European commitments looming, Barcelona will need to adapt quickly to maintain their momentum without one of their defensive linchpins.
Manchester United have reportedly made a bold move to sign Bayer Leverkusen's Jonathan Tah, sensing a chance to hijack the defender's expected transfer to Barcelona, according to Sky Germany.
Barcelona's Marc-Andre ter Stegen is set to reclaim his spot between the posts for the Catalans' clash against Real Valladolid on Saturday, manager Hansi Flick confirmed on Friday.
Manchester City battled to a hard-fought 1-0 victory against Wolverhampton Wanderers at the Etihad Stadium, with Kevin De Bruyne's first-half strike proving decisive.
In a pulsating UEFA Champions League semi-final first leg, Barcelona and Inter Milan played out a breathtaking 3-3 draw at the Lluis Companys Olympic Stadium, with teenage sensation Lamine Yamal stealing the show.
