USA needed four-and-a-half points from Sunday's 12 singles matches to retain the trophy at Royal Montreal Golf Club.
Ryder Cup captain Keegan Bradley clinched the winning point as the International team remained without a victory in the event since 1998.
Xander Schauffele comprehensively beat Jason Day 4&3 in the opening clash, while Russell Henley and Patrick Cantlay also won their matches and Sam Burns halved his contest with Tom Kim.
That allowed Bradley to clinch the trophy as he completed a one-up victory over Kim Si-woo.
Bradley said in an interview on the PGA Tour's website: "Wow, that was incredible. I didn't know if I would get to do this again and to be able to just play in this tournament, and to win the point… my goodness.
"The last time I played, I was the point to lose the Ryder Cup. If this is my last round as a player -maybe it is - I'm happy with that."
Collin Morikawa and Max Homa also recorded wins for the US, while Hideki Matsuyama, Corey Conners and Christiaan Bezuidenhout were victors for the International team.
The Internationals will once again attempt to end their decades-long drought at this week's Presidents Cup, while the United States will look to extend its remarkable run of dominance.
Jim Furyk and Keegan Bradley have been named assistant captains for the upcoming Presidents Cup, providing US captain Brandt Snedeker with additional experience ahead of the event at Medinah in September.
Chris Gotterup defeated Hideki Matsuyama on the first playoff hole to claim his fourth PGA Tour title at the Phoenix Open on Sunday.
Hideki Matsuyama will take a slender one-stroke lead into the final round of the Phoenix Open on Sunday.
