Sudanese outfit Al Hilal complete Group C but all eyes will be on Mokwena when he faces his former club with his Algeria team.
Lupopo knocked out the Buccaneers on penalties in the preliminary round after a thrilling 6-6 draw over two legs and will now return to South Africa during the group stage.
Pirates' elimination means Sundowns will be the only South African side in the continent's biggest club tournament this season.
The top two sides in each of the four groups will progress to the quarter-finals.
Defending champions from Egypt, Pyramids FC, were drawn in Group A alongside RS Berkane, Rivers United and Power Dynamos.
Mamelodi Sundowns head coach Miguel Cardoso believes South African football must urgently improve its structures and scheduling if local clubs are to regularly challenge for continental honours.
Teboho Mokoena could barely contain his emotions after helping Mamelodi Sundowns secure the CAF Champions League title for the second time in the club's history on Sunday night.
Teboho Mokoena’s stunning first-half strike proved decisive as Mamelodi Sundowns secured a 2-1 aggregate victory over AS FAR to win the CAF Champions League in Morocco on Sunday night.
Mamelodi Sundowns defender Divine Lunga has been officially released from Zimbabwe’s Unity Cup squad in order to focus on the CAF Champions League final second leg.
