The Eagles of Carthage face Japan, the Netherlands and Sweden in Group F when the tournament kicks off in June.
Lamouchi guided his side to the global showpiece as winners of Group H ahead of Nambia, Liberia, Malawi, Equatorial Guinea and Sao Tome and Principe.
Included in the squad are Paris Saint-Germain winger Khalil Ayari, Union Berlin midfielder Rani Khedira, Frankfurt midfielder Ellyes Skhiri and Burnley's Hannibal Mejbri.
Missing out though are the likes of experienced Al-Gharafa midfielder Ferjani Sassi, former captain Yassine Meriah and veteran winger Naim Sliti.
Full Tunisia World Cup squad:
Goalkeepers: Aymen Dahmen (CS Sfaxien), Sabri Ben Hassine (Étoile du Sahel), Mohib Al-Shamikhi (Club Africain)
Defenders: Montassar Talbi (Lorient), Dylan Bronn (Servette), Omar Rekik (Maribor), Yan Valery (Young Boys), Ali Abdi (Nice), Moataz Nafati (IFK Norrköping), Raed Sheikhawi (US Monastir), Adem Arous (Kasimpasa)
Midfielders: Ellyes Skhiri (Eintracht Frankfurt), Hannibal Mejbri (Burnley), Amine Ben Hamida (Espérance de Tunis), Anis Ben Slimane (Norwich City), Mohamed Haj Mahmoud (Lugano), Rani Khedira (Union Berlin), Mortadha Ben Ouanes (Kasimpasa)
Forwards: Elias Achouri (Copenhagen), Ismael Gharbi (FC Augsburg), Elyes Saad (Hannover 96), Sebastian Tounekti (Celtic), Firas Chaouat (Club Africain), Khalil Ayari (Paris Saint-Germain), Hazem Mestouri (Dynamo Makhachkala), Rayan Loumi (Vancouver Whitecaps)
Germany head coach Julian Nagelsmann refused to confirm or deny rumours suggesting Manuel Neuer was set to return from international retirement to join Germany's 2026 FIFA World Cup squad.
Argentina have named a provisional 55-man squad for the upcoming 2026 FIFA World Cup.
New Ghana head coach Carlos Queiroz has moved to motivate his charges ahead of their participation at the FIFA 2026 World Cup.
Brazil forward Endrick has said the support of Jude Bellingham helped him through a difficult period at Real Madrid as he continues chasing his dream of playing for Brazil at the FIFA World Cup.
